RKM-2018-003

RSP 11094

Grower: R-Kiem Seeds

General Information

Accession Date
September 6, 2018
Reported Plant Sex
Female
DNA Extracted From
Stem

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 0.82%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0390
male female RSP11094

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

No variants to report

Variants (Select Genes of Interest)

PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.419A>G p.Asp140Gly missense variant moderate contig700 2721254

IGV: Start, Jump

T/C
NGS:
0.430
C90:
0.440
DXR-1

UniProt

c.139A>G p.Arg47Gly missense variant moderate contig380 235296

IGV: Start, Jump

T/C
NGS:
0.020
C90:
0.000
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.058 0.117 0.175 0.233
closely related moderately related distantly related
  1. 0.036 Cheese (RSP10460)
  2. 0.161 Northern Lights (RSP11501)
  3. 0.184 RKM-2018-020 (RSP11112)
  4. 0.191 Black Jack (RSP10603)
  5. 0.196 BLACK JACK (RSP11346)
  6. 0.197 RKM-2018-025 (RSP11117)
  7. 0.201 Italian Kiss (RSP10990)
  8. 0.202 RKM-2018-016 (RSP11108)
  9. 0.206 Italian Kiss (RSP11034)
  10. 0.206 Top 44 (SRR14708268)
  11. 0.212 Rest (RSP11377)
  12. 0.215 Durban Poison #1 (RSP10996)
  13. 0.219 RKM-2018-027 (RSP11119)
  14. 0.220 RKM-2018-030 (RSP11122)
  15. 0.220 Gold Cracker (RSP11041)
  16. 0.221 Serious Happiness (RSP10763)
  17. 0.221 Electra (RSP11366)
  18. 0.221 Blue Dream (RSP11004)
  19. 0.223 Sour D (RSP11343)
  20. 0.224 Up Sunset (RSP11256)

Most genetically distant strains (All Samples)

0 0.117 0.233 0.350 0.467
closely related moderately related distantly related
  1. 0.439 Cherry Blossom (RSP11314)
  2. 0.430 Cherry Blossom (RSP11323)
  3. 0.429 Cherry Blossom (RSP11311)
  4. 0.420 Cherry Blossom (RSP11333)
  5. 0.420 Feral (RSP11205)
  6. 0.418 Cherry Blossom (RSP11318)
  7. 0.416 Cherry Blossom (RSP11334)
  8. 0.414 Cherry Blossom (RSP11317)
  9. 0.411 80E (RSP11213)
  10. 0.405 CS (RSP11208)
  11. 0.402 Fatso (RSP11741)
  12. 0.400 Cherry Blossom (RSP11322)
  13. 0.398 Absolute OG (RSP11455)
  14. 0.398 Carmaleonte (RSP11207)
  15. 0.397 Cherry Blossom (RSP11330)
  16. 0.395 80E (RSP11211)
  17. 0.395 Feral (RSP10890)
  18. 0.394 Cherry Blossom (RSP11326)
  19. 0.394 Feral (RSP11206)
  20. 0.393 Cherry Blossom (RSP11306)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4448559
Overlapping SNPs:
124
Concordance:
113

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495180
Overlapping SNPs:
8
Concordance:
8

Blockchain Registration Information

Transaction ID
26c1172cbea3c38ec17cffb9af4bd35aae5f994a7d49d4d8385571c8b2539a20
Stamping Certificate
Download PDF (857.0 KB)
SHASUM Hash
5c0ef2eeb82aba530f5741572ff275ffb53d07f5ae1ce84e13c2fea3cd764080
QR code for RSP11094

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