Cbot-2019-002

RSP 11130

Grower: Research Development Cannabinoid

General Information

Accession Date
February 5, 2019
Reported Plant Sex
not reported
DNA Extracted From
Stem

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type III

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.02%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0381
male female RSP11130

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

No variants to report

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.934C>G p.His312Asp missense variant moderate contig700 1950704

IGV: Start, Jump

G/C
NGS:
0.410
C90:
0.000
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-2b

UniProt

c.-2_1dupATA start lost & conservative inframe insertion high contig700 1951880

IGV: Start, Jump

A/ATAT
NGS:
0.410
C90:
0.000
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.492G>C p.Glu164Asp missense variant moderate contig700 2721181

IGV: Start, Jump

C/G
NGS:
0.048
C90:
0.000
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.419A>G p.Asp140Gly missense variant moderate contig700 2721254

IGV: Start, Jump

T/C
NGS:
0.430
C90:
0.440
PKSG-4b

UniProt

c.352_355delACAG p.Thr118fs frameshift variant high contig700 2721317

IGV: Start, Jump

CCTGT/C
NGS:
0.529
C90:
0.000
PKSG-4b

UniProt

c.323A>G p.Glu108Gly missense variant moderate contig700 2721350

IGV: Start, Jump

T/C
NGS:
0.469
C90:
0.000
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.067 0.133 0.200 0.267
closely related moderately related distantly related
  1. 0.166 Cbot-2019-004 (RSP11132)
  2. 0.186 Cbot-2019-003 (RSP11131)
  3. 0.206 Liberty Haze (RSP11000)
  4. 0.216 Blueberry Cheesecake (RSP10684)
  5. 0.217 Durban Poison #1 (RSP11013)
  6. 0.220 Charlotte Dream (RSP11412)
  7. 0.221 Joy (RSP11380)
  8. 0.224 Durban Poison #1 (RSP10996)
  9. 0.224 Electra (RSP11366)
  10. 0.224 Durban Poison (RSP11014)
  11. 0.226 13 Cherries (RSP11442)
  12. 0.226 Strawnana (RSP12094)
  13. 0.229 Sparrow (RSP11443)
  14. 0.230 Domnesia (RSP11184)
  15. 0.230 JL Cross 11 (RSP11512)
  16. 0.233 Trump x Trump (RSP11466)
  17. 0.233 RAPHAEL #2 (RSP11172)
  18. 0.234 Durban Poison (RSP10998)
  19. 0.234 Lifter (RSP11365)
  20. 0.236 Suver Haze (RSP11364)

Most genetically distant strains (All Samples)

0 0.108 0.217 0.325 0.433
closely related moderately related distantly related
  1. 0.417 JL yellow (RSP11075)
  2. 0.416 Northern Skunk (RSP11456)
  3. 0.408 JL 3rd Gen Mother (RSP11214)
  4. 0.407 Cherry Blossom (RSP11318)
  5. 0.404 JL 3rd Gen Father (RSP11196)
  6. 0.397 Cherry Blossom (RSP11333)
  7. 0.393 R1in136 (SRR14708226)
  8. 0.392 Cherry Blossom (RSP11317)
  9. 0.392 Eran Almog 05MAY2017 (RSP10937)
  10. 0.391 Cherry Blossom (RSP11314)
  11. 0.387 Cherry Blossom (RSP11311)
  12. 0.383 JL 3rd Gen Mother (RSP11197)
  13. 0.381 GMO x Garlic Breath (RSP12507)
  14. 0.380 80E (RSP11213)
  15. 0.380 Cherry Blossom (RSP11301)
  16. 0.380 Cherry Blossom (RSP11324)
  17. 0.379 Ruderalis Indica (SRR14708267)
  18. 0.379 Cherry Blossom (RSP11328)
  19. 0.378 JL 4th Gen 2 (RSP11194)
  20. 0.377 JL 4th Gen 1 (RSP11193)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4450103
Overlapping SNPs:
52
Concordance:
33

Nearest genetic relative in Lynch dataset

Lynch Strain
Overlapping SNPs:
3
Concordance:
3

Blockchain Registration Information

Transaction ID
b9f97aa42a60dd0dc7de24ac7d0118e85ef97eebb6f357b1a584073dffad9ee8
Stamping Certificate
Download PDF (855.6 KB)
SHASUM Hash
b714d82bfe0b7d9a70a987cab1da025be8a13ba2ace8beb6cfde30f4e3cc8d45
QR code for RSP11130

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