Durban Poison

RSP 10998

Grower: DigiPath Labs

General Information

Sample Name
F20161209-03-8
Accession Date
August 28, 2017
Reported Plant Sex
Female
DNA Extracted From
Unknown

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

THC + THCA
23.3%
CBD + CBDA
0.035%
THCV + THCVA
0.038%
CBC + CBCA
0.019%
CBG + CBGA
0.85%
CBN + CBNA
0.146%

Terpenoids

α-Bisabolol
0.001%
Borneol
n/a
Camphene
0.002%
Carene
0.102%
Caryophyllene oxide
0.001%
β-Caryophyllene
0.065%
Fenchol
n/a
Geraniol
0.006%
α-Humulene
0.063%
Limonene
0.084%
Linalool
0.005%
Myrcene
0.174%
α-Phellandrene
n/a
Terpinolene
0.585%
α-Terpineol
n/a
α-Terpinene
0.049%
γ-Terpinene
0.843%
Total Nerolidol
0.005%
Total Ocimene
0.283%
α-Pinene
0.118%
β-Pinene
0.194%

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.03%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0449
male female RSP10998

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

No variants to report

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
aPT4

UniProt

c.1168T>C p.Tyr390His missense variant moderate contig121 2833503

IGV: Start, Jump

T/C
NGS:
0.546
C90:
0.000
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.050 0.100 0.150 0.200
closely related moderately related distantly related
  1. 0.002 Durban Poison (RSP11226)
  2. 0.006 Durban Poison #1 (RSP11013)
  3. 0.007 Durban Poison #1 (RSP10996)
  4. 0.007 Durban Poison (RSP11014)
  5. 0.135 Black Jack (RSP10603)
  6. 0.147 RKM-2018-025 (RSP11117)
  7. 0.147 BLACK JACK (RSP11346)
  8. 0.148 RKM-2018-016 (RSP11108)
  9. 0.149 Domnesia (RSP11184)
  10. 0.172 Lift (RSP11378)
  11. 0.172 Gold Cracker (RSP11048)
  12. 0.175 Electra (RSP11366)
  13. 0.176 Saint Jack (RSP11179)
  14. 0.182 RKM-2018-027 (RSP11119)
  15. 0.184 Serious Happiness (RSP10763)
  16. 0.188 Gold Cracker (RSP11041)
  17. 0.190 Cheese (RSP10460)
  18. 0.192 UnObtanium (RSP11611)
  19. 0.196 Doug s Varin (RSP11243)
  20. 0.199 Tangerine Haze (RSP10995)

Most genetically distant strains (All Samples)

0 0.108 0.217 0.325 0.433
closely related moderately related distantly related
  1. 0.429 Cherry Blossom (RSP11323)
  2. 0.411 80E (RSP11213)
  3. 0.400 Cherry Blossom (RSP11314)
  4. 0.392 80E (RSP11212)
  5. 0.391 Cherry Blossom (RSP11318)
  6. 0.387 Cherry Blossom (RSP11317)
  7. 0.383 Cherry Blossom (RSP11311)
  8. 0.381 80E (RSP11211)
  9. 0.380 Cherry Blossom (RSP11274)
  10. 0.378 CS (RSP11208)
  11. 0.378 Feral (RSP11205)
  12. 0.375 IUP2 (SRR14708257)
  13. 0.375 Cherry Blossom (RSP11306)
  14. 0.374 Cherry Blossom (RSP11333)
  15. 0.374 Kush Hemp E1 (RSP11128)
  16. 0.374 Feral (RSP10891)
  17. 0.370 IUP3 (SRR14708256)
  18. 0.369 R1in136 (SRR14708227)
  19. 0.368 Tanao Sri -46- (RSP11486)
  20. 0.367 Carmaleonte (RSP11207)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8346957
Overlapping SNPs:
99
Concordance:
95

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495198
Overlapping SNPs:
11
Concordance:
10

Blockchain Registration Information

Transaction ID
0227f127b2679f9f055219de913e02a45807410093689377997334bb2cdc3151
Stamping Certificate
Download PDF (842.4 KB)
SHASUM Hash
bdfa4c953a59ff0b1fc56c4df04ede8867e694ec210617f06e87b72409e68c6a
QR code for RSP10998

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