JL#2

RSP 11076

Grower: emerald pharms

General Information

Accession Date
June 12, 2018
Reported Plant Sex
Female
DNA Extracted From
Stem

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type II

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 0.8%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0451
male female RSP11076

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.998C>G p.Pro333Arg missense variant moderate contig741 4416830

IGV: Start, Jump

G/C
NGS:
0.182
C90:
0.000

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.934C>G p.His312Asp missense variant moderate contig700 1950704

IGV: Start, Jump

G/C
NGS:
0.410
C90:
0.000
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.492G>C p.Glu164Asp missense variant moderate contig700 2721181

IGV: Start, Jump

C/G
NGS:
0.048
C90:
0.000
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.484_485insGG p.Lys162fs frameshift variant high contig700 2721188

IGV: Start, Jump

T/TCC
NGS:
0.002
C90:
0.000
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.419A>G p.Asp140Gly missense variant moderate contig700 2721254

IGV: Start, Jump

T/C
NGS:
0.430
C90:
0.440
DXR-1

UniProt

c.139A>G p.Arg47Gly missense variant moderate contig380 235296

IGV: Start, Jump

T/C
NGS:
0.020
C90:
0.000
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.075 0.150 0.225 0.300
closely related moderately related distantly related
  1. 0.039 Blueberry Cheesecake (RSP10670)
  2. 0.040 Blueberry Cheesecake (RSP10672)
  3. 0.043 Blueberry Cheesecake (RSP10671)
  4. 0.210 BlueBerry Cheesecake x JL Male (RSP11201)
  5. 0.242 JL Compost (RSP11657)
  6. 0.277 Durban Poison (RSP11014)
  7. 0.279 RKM-2018-031 (RSP11123)
  8. 0.282 Chematonic -Cannatonic x Chemdawg- (RSP11394)
  9. 0.283 Joy (RSP11380)
  10. 0.284 Cherry (RSP11143)
  11. 0.284 Blueberry Cheesecake (RSP10684)
  12. 0.293 Durban Poison #1 (RSP11013)
  13. 0.294 Queen Jesus (RSP10105)
  14. 0.296 CST (RSP11002)
  15. 0.297 Green Crack (RSP11339)
  16. 0.299 Midnight 05MAY2017 (RSP10941)
  17. 0.299 Black Jack (RSP10603)
  18. 0.300 Cbot-2019-001 (RSP11129)
  19. 0.300 Cbot-2019-003 (RSP11131)
  20. 0.300 Calm (RSP11379)

Most genetically distant strains (All Samples)

0 0.117 0.233 0.350 0.467
closely related moderately related distantly related
  1. 0.459 Cherry Blossom CBG (RSP11303)
  2. 0.448 Cherry Blossom (RSP11314)
  3. 0.441 Big Bud (SRR14708270)
  4. 0.432 Cherry Blossom (RSP11333)
  5. 0.431 RKM-2018-026 (RSP11118)
  6. 0.427 Cherry Blossom (RSP11334)
  7. 0.427 Cherry Blossom (RSP11317)
  8. 0.424 Cherry Blossom (RSP11306)
  9. 0.420 Fatso (RSP11741)
  10. 0.420 Cherry Blossom (RSP11324)
  11. 0.419 RKM-2018-012 (RSP11103)
  12. 0.417 GG4 (RSP11978)
  13. 0.416 Cherry Blossom (RSP11328)
  14. 0.415 Feral (RSP11205)
  15. 0.415 Cherry Blossom (RSP11309)
  16. 0.414 80E (RSP11213)
  17. 0.413 GMO x Poison Momosa (RSP12626)
  18. 0.413 SourD (RSP12092)
  19. 0.413 Cherry Blossom (RSP11329)
  20. 0.413 NSPM x NSPM (RSP11487)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8347130
Overlapping SNPs:
121
Concordance:
76

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495248
Overlapping SNPs:
3
Concordance:
3

Blockchain Registration Information

Transaction ID
4f64ea7bb855c0aa4c67d221d1ed2677f3158d347b13167414af5f629fdd8a46
Stamping Certificate
Download PDF (843.9 KB)
SHASUM Hash
44fd5a8264eaf0a7a8dd4eca66b001b33532ef652f2e15a0562490b56be09dba
QR code for RSP11076

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