Dave Alaska

RSP 11627

Grower: Denise Marston

General Information

Accession Date
July 28, 2020
Reported Plant Sex
Female
DNA Extracted From
Stem

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.02%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0407
male female RSP11627

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.749C>A p.Ala250Asp missense variant moderate contig741 4417079

IGV: Start, Jump

G/T
NGS:
0.127
C90:
0.632

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.1105C>G p.Pro369Ala missense variant moderate contig700 1944285

IGV: Start, Jump

G/C
NGS:
0.011
C90:
0.000
PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1124G>C p.Arg375Thr missense variant moderate contig700 1950514

IGV: Start, Jump

C/G
NGS:
0.015
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.774G>A p.Met258Ile missense variant moderate contig700 1950864

IGV: Start, Jump

C/T
NGS:
0.366
C90:
0.947
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.353_354insCC p.Gly119fs frameshift variant high contig700 2721319

IGV: Start, Jump

T/TGG
NGS:
0.175
C90:
0.000
DXR-1

UniProt

c.20G>T p.Cys7Phe missense variant moderate contig380 235947

IGV: Start, Jump

C/A
NGS:
0.020
C90:
0.000
DXR-2

UniProt

c.1319T>C p.Ile440Thr missense variant moderate contig380 285250

IGV: Start, Jump

A/G
NGS:
0.480
C90:
0.000
DXR-2

UniProt

c.1140A>C p.Lys380Asn missense variant moderate contig380 285582

IGV: Start, Jump

T/G
NGS:
0.018
C90:
0.000
FAD2-2

UniProt

c.58C>T p.His20Tyr missense variant moderate contig83 1803311

IGV: Start, Jump

G/A
NGS:
0.013
C90:
0.000
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000
HDS-1

UniProt

c.-108+1_-108+2insG splice donor variant & intron variant high contig1891 889975

IGV: Start, Jump

A/AC
NGS:
0.217
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.058 0.117 0.175 0.233
closely related moderately related distantly related
  1. 0.140 Sour D (RSP11343)
  2. 0.148 East Coast Sour Diesel (RSP10243)
  3. 0.153 New York City Deisel (RSP11225)
  4. 0.171 RKM-2018-002 (RSP11093)
  5. 0.198 Trump x Trump (RSP11466)
  6. 0.207 Blueberry Cheesecake (RSP10684)
  7. 0.210 Hermaphrodite Research Sample1 (RSP11042)
  8. 0.213 Hermaphrodite Research Sample1 (RSP11049)
  9. 0.213 501st OG (RSP11241)
  10. 0.213 Durban Poison #1 (RSP11013)
  11. 0.214 SourD (RSP12092)
  12. 0.215 Dog Patch (RSP11725)
  13. 0.216 Thank You Jerry (RSP11459)
  14. 0.217 Durban Poison #1 (RSP10996)
  15. 0.219 Lime OG (RSP12101)
  16. 0.220 Whitey (RSP11363)
  17. 0.220 Domnesia (RSP11184)
  18. 0.221 CPH (RSP11367)
  19. 0.222 Star Dawg (RSP11352)
  20. 0.223 Pie Hoe (RSP11073)

Most genetically distant strains (All Samples)

0 0.125 0.250 0.375 0.500
closely related moderately related distantly related
  1. 0.468 80E (RSP11213)
  2. 0.447 80E (RSP11211)
  3. 0.441 80E (RSP11212)
  4. 0.440 Tiger Tail -30- (RSP11484)
  5. 0.426 Cherry Blossom (RSP11306)
  6. 0.426 Cherry Blossom (RSP11311)
  7. 0.425 Cherry Blossom (RSP11301)
  8. 0.424 Cherry Blossom (RSP11314)
  9. 0.424 JL yellow (RSP11075)
  10. 0.420 Northern Skunk (RSP11456)
  11. 0.420 Cherry Blossom (RSP11322)
  12. 0.420 Cherry Blossom (RSP11300)
  13. 0.417 Cherry Blossom (RSP11318)
  14. 0.416 JL 3rd Gen Mother (RSP11214)
  15. 0.416 Tanao Sri -46- (RSP11486)
  16. 0.415 IUP3 (SRR14708256)
  17. 0.414 Cherry Blossom (RSP11324)
  18. 0.413 Cherry Blossom (RSP11333)
  19. 0.413 Feral (RSP11205)
  20. 0.413 IUP2 (SRR14708257)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4448401
Overlapping SNPs:
88
Concordance:
61

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495314
Overlapping SNPs:
3
Concordance:
3

Blockchain Registration Information

SHASUM Hash
1277f504b7a63def3ae5d5291175a9ee0595f81e078c1096ca6c8f9c262b0917
QR code for RSP11627

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