Advanced Strain Search

A15

RSP 12655

Grower: Humboldt DNA

General Information

Accession Date
July 20, 2022
Reported Plant Sex
Male
DNA Extracted From
Stem

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

File Downloads

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.16%

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0921

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)
THCAS c.998C>G p.Pro333Arg missense variant moderate contig741 4416830

IGV: Start, Jump

G/C
NGS:
0.181
C90:
0.000
THCAS c.749C>A p.Ala250Asp missense variant moderate contig741 4417079

IGV: Start, Jump

G/T
NGS:
0.399
C90:
0.632

Variants (Select Genes of Interest)
PHL-2 c.455A>C p.Asp152Ala missense variant moderate contig2621 339191

IGV: Start, Jump

A/C
NGS:
0.239
C90:
0.507
PHL-2 c.2564T>A p.Phe855Tyr missense variant moderate contig2621 342607

IGV: Start, Jump

T/A
NGS:
0.483
C90:
0.732
PHL-2 c.2578T>A p.Leu860Ile missense variant moderate contig2621 342621

IGV: Start, Jump

T/A
NGS:
0.486
C90:
0.746
PHL-2 c.2756A>C p.Glu919Ala missense variant moderate contig2621 342799

IGV: Start, Jump

A/C
NGS:
0.409
C90:
0.675
PHL-2 c.2783G>A p.Ser928Asn missense variant moderate contig2621 342826

IGV: Start, Jump

G/A
NGS:
0.611
C90:
0.890
PHL-2 c.2830A>G p.Asn944Asp missense variant moderate contig2621 342873

IGV: Start, Jump

A/G
NGS:
0.146
C90:
0.000
PHL-2 c.2903_2905dupGCA p.Ser968dup disruptive inframe insertion moderate contig2621 342939

IGV: Start, Jump

T/TGCA
NGS:
0.239
C90:
0.522
PHL-2 c.3209A>G p.Gln1070Arg missense variant moderate contig2621 343252

IGV: Start, Jump

A/G
NGS:
0.599
C90:
0.885
PHL-2 c.3467A>G p.Gln1156Arg missense variant moderate contig2621 343510

IGV: Start, Jump

A/G
NGS:
0.487
C90:
0.713
PHL-2 c.3552delG p.Lys1185fs frameshift variant high contig2621 343593

IGV: Start, Jump

CG/C
NGS:
0.131
C90:
0.000
DXR-2

UniProt

c.1319T>C p.Ile440Thr missense variant moderate contig380 285250

IGV: Start, Jump

A/G
NGS:
0.454
C90:
0.000
DXR-2

UniProt

c.431C>G p.Ala144Gly missense variant moderate contig380 287760

IGV: Start, Jump

G/C
NGS:
0.437
C90:
0.550
FAD2-2

UniProt

c.164A>G p.His55Arg missense variant moderate contig83 1803205

IGV: Start, Jump

T/C
NGS:
0.050
C90:
0.000
FAD2-2

UniProt

c.161T>A p.Leu54His missense variant moderate contig83 1803208

IGV: Start, Jump

A/T
NGS:
0.337
C90:
0.000
FAD2-2

UniProt

c.64G>T p.Ala22Ser missense variant moderate contig83 1803305

IGV: Start, Jump

C/A
NGS:
0.080
C90:
0.000
ELF3

UniProt

c.358G>A p.Gly120Arg missense variant moderate contig97 242064

IGV: Start, Jump

G/A
NGS:
0.582
C90:
0.493
ELF3

UniProt

c.520A>C p.Asn174His missense variant moderate contig97 242226

IGV: Start, Jump

A/C
NGS:
0.567
C90:
0.502
ELF3

UniProt

c.752G>T p.Gly251Val missense variant moderate contig97 242458

IGV: Start, Jump

G/T
NGS:
0.091
C90:
0.139
ELF3

UniProt

c.772A>G p.Ser258Gly missense variant moderate contig97 242478

IGV: Start, Jump

A/G
NGS:
0.386
C90:
0.000
ELF3

UniProt

c.812G>C p.Gly271Ala missense variant moderate contig97 242518

IGV: Start, Jump

G/C
NGS:
0.649
C90:
0.938
ELF3

UniProt

c.1466G>A p.Ser489Asn missense variant moderate contig97 244297

IGV: Start, Jump

G/A
NGS:
0.385
C90:
0.000
ELF3

UniProt

c.1630A>G p.Thr544Ala missense variant moderate contig97 244461

IGV: Start, Jump

A/G
NGS:
0.570
C90:
0.545
ELF3

UniProt

c.1803_1805delTCA p.His601del disruptive inframe deletion moderate contig97 244625

IGV: Start, Jump

ACAT/A
NGS:
0.342
C90:
0.000
ELF3

UniProt

c.1966C>G p.Pro656Ala missense variant moderate contig97 244797

IGV: Start, Jump

C/G
NGS:
0.627
C90:
0.632
ELF3

UniProt

c.2141C>G p.Pro714Arg missense variant moderate contig97 244972

IGV: Start, Jump

C/G
NGS:
0.507
C90:
0.335
ELF3

UniProt

c.2198G>T p.Arg733Leu missense variant moderate contig97 245029

IGV: Start, Jump

G/T
NGS:
0.620
C90:
0.531
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.544
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.522
C90:
0.000
PHL-1

UniProt

c.2651C>T p.Ala884Val missense variant moderate contig1439 1487146

IGV: Start, Jump

G/A
NGS:
0.172
C90:
0.622
PHL-1

UniProt

c.2623A>G p.Thr875Ala missense variant moderate contig1439 1487174

IGV: Start, Jump

T/C
NGS:
0.405
C90:
0.000
PHL-1

UniProt

c.2561A>T p.Asn854Ile missense variant moderate contig1439 1487236

IGV: Start, Jump

T/A
NGS:
0.165
C90:
0.612
PHL-1

UniProt

c.2551A>G p.Thr851Ala missense variant moderate contig1439 1487246

IGV: Start, Jump

T/C
NGS:
0.563
C90:
0.890
PHL-1

UniProt

c.1387A>G p.Thr463Ala missense variant moderate contig1439 1489811

IGV: Start, Jump

T/C
NGS:
0.570
C90:
0.904
Edestin

UniProt

c.16T>C p.Ser6Pro missense variant moderate contig850 3065274

IGV: Start, Jump

A/G
NGS:
0.152
C90:
0.000
HDS-1

UniProt

c.1618A>G p.Ile540Val missense variant moderate contig1891 885936

IGV: Start, Jump

T/C
NGS:
0.584
C90:
0.861
HDS-1

UniProt

c.56C>G p.Ala19Gly missense variant moderate contig1891 889336

IGV: Start, Jump

G/C
NGS:
0.648
C90:
0.943
HDS-1

UniProt

c.35G>A p.Cys12Tyr missense variant moderate contig1891 889357

IGV: Start, Jump

C/T
NGS:
0.481
C90:
0.646
PIE1-2

UniProt

c.6773T>C p.Leu2258Ser missense variant moderate contig1460 1184314

IGV: Start, Jump

A/G
NGS:
0.313
C90:
0.675
PIE1-2

UniProt

c.6623C>T p.Ala2208Val missense variant moderate contig1460 1184464

IGV: Start, Jump

G/A
NGS:
0.281
C90:
0.689
PIE1-2

UniProt

c.1872T>A p.Asp624Glu missense variant moderate contig1460 1190252

IGV: Start, Jump

A/T
NGS:
0.460
C90:
0.990
PIE1-2

UniProt

c.1289A>G p.Asp430Gly missense variant moderate contig1460 1192109

IGV: Start, Jump

T/C
NGS:
0.214
C90:
0.000
EMF2

UniProt

c.1772A>G p.Gln591Arg missense variant moderate contig954 3059929

IGV: Start, Jump

A/G
NGS:
0.584
C90:
0.876
FLD

UniProt

c.2981T>C p.Met994Thr missense variant moderate contig1450 2044012

IGV: Start, Jump

A/G
NGS:
0.494
C90:
0.478
FLD

UniProt

c.2964C>A p.Asp988Glu missense variant moderate contig1450 2044029

IGV: Start, Jump

G/T
NGS:
0.477
C90:
0.464
FLD

UniProt

c.2929T>C p.Phe977Leu missense variant moderate contig1450 2044103

IGV: Start, Jump

A/G
NGS:
0.332
C90:
0.349
FLD

UniProt

c.125G>A p.Ser42Asn missense variant moderate contig1450 2047909

IGV: Start, Jump

C/T
NGS:
0.382
C90:
0.388
PIE1-1

UniProt

c.100G>A p.Glu34Lys missense variant moderate contig1225 2277786

IGV: Start, Jump

G/A
NGS:
0.251
C90:
0.478
PIE1-1

UniProt

c.1222C>G p.Gln408Glu missense variant moderate contig1225 2281482

IGV: Start, Jump

C/G
NGS:
0.542
C90:
0.852
PIE1-1

UniProt

c.6725C>T p.Ala2242Val missense variant moderate contig1225 2289290

IGV: Start, Jump

C/T
NGS:
0.359
C90:
0.737
PIE1-1

UniProt

c.6875T>C p.Leu2292Ser missense variant moderate contig1225 2289440

IGV: Start, Jump

T/C
NGS:
0.199
C90:
0.679
GGR

UniProt

c.704A>T p.His235Leu missense variant moderate contig2282 549696

IGV: Start, Jump

A/T
NGS:
0.382
C90:
0.000

Nearest genetic relatives (All Samples)

closely related moderately related distantly related
  1. 0.141 A13 (RSP12654)
  2. 0.195 S21-002-01 (RSP12656)
  3. 0.206 JL X NSPM1 7 (RSP11469)
  4. 0.208 SHERBERT (RSP11355)
  5. 0.209 JL Cross 10 (RSP11511)
  6. 0.212 JL Tent 1 yellow stake (RSP11488)
  7. 0.213 Skywalker OG (RSP10837)
  8. 0.215 JL Cross 23 (RSP11524)
  9. 0.215 SFVxTK (RSP11072)
  10. 0.216 RKM-2018-033 (RSP11125)
  11. 0.219 Mother s Milk No 31 (RSP11623)
  12. 0.219 NSPM1 (RSP11362)
  13. 0.220 unknown (RSP11432)
  14. 0.220 JL Cross 22 (RSP11523)
  15. 0.221 Mendo Purple (RSP11494)
  16. 0.221 Garlic (RSP11341)
  17. 0.222 501st OG (RSP11241)
  18. 0.222 Rootbeer Rolex 50 (RSP12645)
  19. 0.224 JL x NSPM1 1 5 (RSP11479)
  20. 0.225 GMO (RSP12091)

Most genetically distant strains (All Samples)

closely related moderately related distantly related
  1. 0.460 Cherry Blossom (RSP11311)
  2. 0.448 Cherry Blossom (RSP11323)
  3. 0.444 Northern Skunk (RSP11456)
  4. 0.442 Cherry Blossom (RSP11328)
  5. 0.439 Cherry Blossom (RSP11318)
  6. 0.436 Unknown- Cherry Wine - 001 (RSP11268)
  7. 0.433 Tiger Tail 30 (RSP11484)
  8. 0.429 Cherry Blossom (RSP11314)
  9. 0.424 Cherry Blossom (RSP11317)
  10. 0.424 Cherry Blossom (RSP11333)
  11. 0.420 Cherry Blossom (RSP11334)
  12. 0.420 Unknown- Cherry Wine - 003 (RSP11270)
  13. 0.419 CS (RSP11208)
  14. 0.418 Northern Lights (RSP11501)
  15. 0.415 Cherry Blossom (RSP11324)
  16. 0.415 Cbot-2019-005 (RSP11133)
  17. 0.414 Tanao Sri 46 (RSP11486)
  18. 0.413 Cherry Blossom (RSP11301)
  19. 0.413 JL 3rd Gen Father (RSP11196)
  20. 0.412 Cherry Blossom (RSP11325)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8347128
Overlapping SNPs:
86
Concordance:
54

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495313
Overlapping SNPs:
8
Concordance:
8

Blockchain Registration Information

Transaction ID
36a2b637a8185039fcfcfc57a157ef991329d2a6da081477b4483a631f869f5d
Stamping Certificate
Download PDF (39.5 KB)
SHASUM Hash
d007ac1280c94c1969ad7f542877ef84a8c0e23977bc9f0259235dbd5c3560c8
QR code for RSP12655

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