Golden Road

RSP 11345

Grower: Happy Valley

Summary

Golden Road (RSP11345/Happy Valley) is a genetic Type I plant and its genetics appear rare. The sample's closest relative in the database is Durban Poison #1 (RSP11013/DigiPath Labs) and it is a potential cousin. The heterozygosity rate is 1.1% which is average (52.0 percentile).

General Information

Accession Date
October 17, 2019
Reported Plant Sex
Female
DNA Extracted From
Stem

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.1%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0504
male female RSP11345

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.749C>A p.Ala250Asp missense variant moderate contig741 4417079

IGV: Start, Jump

G/T
NGS:
0.127
C90:
0.632

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.774G>A p.Met258Ile missense variant moderate contig700 1950864

IGV: Start, Jump

C/T
NGS:
0.366
C90:
0.947
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-4b

UniProt

c.535_545delATTGGAGTGGG p.Ile179fs frameshift variant high contig700 2721127

IGV: Start, Jump

CCCCACTCCAAT/C
NGS:
0.105
C90:
0.000
PKSG-4b

UniProt

c.523C>T p.His175Tyr missense variant moderate contig700 2721150

IGV: Start, Jump

G/A
NGS:
0.112
C90:
0.325
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.353_354insCC p.Gly119fs frameshift variant high contig700 2721319

IGV: Start, Jump

T/TGG
NGS:
0.175
C90:
0.000
DXR-2

UniProt

c.1319T>C p.Ile440Thr missense variant moderate contig380 285250

IGV: Start, Jump

A/G
NGS:
0.480
C90:
0.000
aPT4

UniProt

c.35A>C p.Gln12Pro missense variant moderate contig121 2828691

IGV: Start, Jump

A/C
NGS:
0.075
C90:
0.000
aPT4

UniProt

c.97T>C p.Tyr33His missense variant moderate contig121 2828753

IGV: Start, Jump

T/C
NGS:
0.439
C90:
0.000
aPT4

UniProt

c.153A>C p.Lys51Asn missense variant moderate contig121 2828809

IGV: Start, Jump

A/C
NGS:
0.375
C90:
0.000
aPT4

UniProt

c.216A>T p.Lys72Asn missense variant moderate contig121 2828872

IGV: Start, Jump

A/T
NGS:
0.075
C90:
0.000
aPT4

UniProt

c.775delT p.Tyr259fs frameshift variant high contig121 2831380

IGV: Start, Jump

AT/A
NGS:
0.224
C90:
0.000
aPT4

UniProt

c.1168T>C p.Tyr390His missense variant moderate contig121 2833503

IGV: Start, Jump

T/C
NGS:
0.546
C90:
0.000
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.067 0.133 0.200 0.267
closely related moderately related distantly related
  1. 0.204 Durban Poison #1 (RSP11013)
  2. 0.214 Blueberry Cheesecake (RSP10684)
  3. 0.218 Ringo s Angel (RSP10085)
  4. 0.221 Doug s Varin (RSP11243)
  5. 0.221 Durban Poison #1 (RSP10996)
  6. 0.225 UnObtanium (RSP11611)
  7. 0.226 Trump x Trump (RSP11466)
  8. 0.227 Domnesia (RSP11184)
  9. 0.230 Electra (RSP11366)
  10. 0.231 RKM-2018-004 (RSP11095)
  11. 0.232 RKM-2018-033 (RSP11125)
  12. 0.232 JABBA S STASH (RSP11348)
  13. 0.233 JL Cross 26 (RSP11527)
  14. 0.234 Joy (RSP11380)
  15. 0.234 RKM-2018-004 (RSP11096)
  16. 0.238 Rest (RSP11377)
  17. 0.240 RKM-2018-010 (RSP11101)
  18. 0.241 CBG Berry (RSP11446)
  19. 0.244 JL X NSPM1 7 (RSP11469)
  20. 0.246 Durban Poison (RSP10998)

Most genetically distant strains (All Samples)

0 0.108 0.217 0.325 0.433
closely related moderately related distantly related
  1. 0.421 Cherry Blossom (RSP11318)
  2. 0.411 Cherry Blossom (RSP11317)
  3. 0.411 80E (RSP11213)
  4. 0.409 Cherry Blossom (RSP11306)
  5. 0.403 Cherry Blossom (RSP11322)
  6. 0.403 Cherry Blossom (RSP11333)
  7. 0.403 Cherry Blossom (RSP11323)
  8. 0.393 Cherry Blossom (RSP11324)
  9. 0.390 BagSeed (RSP12627)
  10. 0.387 Wife (RSP11148)
  11. 0.384 CS (RSP11208)
  12. 0.384 Cherry Blossom (RSP11325)
  13. 0.381 CS Indica (RSP11658)
  14. 0.380 Chematonic -Cannatonic x Chemdawg- (RSP11394)
  15. 0.379 Cherry Blossom (RSP11330)
  16. 0.379 Cherry Blossom (RSP11308)
  17. 0.377 Queen Dream CBG (RSP11287)
  18. 0.377 Cherry Blossom (RSP11335)
  19. 0.376 Cherry Blossom (RSP11326)
  20. 0.375 80E (RSP11211)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4451098
Overlapping SNPs:
64
Concordance:
43

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495213
Overlapping SNPs:
6
Concordance:
6

Blockchain Registration Information

Transaction ID
1fc13f1fa36fbf4f83751164dc823c02d7948bdc570331bf98682802f416d265
Stamping Certificate
Download PDF (850.5 KB)
SHASUM Hash
ef8a31b7d50a145b317b25fc1ded075fd513ad6d95bfb21ecafc53d67b66dfa9
QR code for RSP11345

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