T20 Dried

RSP 13125

Grower: Zamir Punja

General Information

Sample Name
T20 Dried-20240416
Accession Date
April 24, 2024
Reported Plant Sex
Female
Microbiome
Krona Plot
Fungal Microbiome
Krona Plot
Bacterial Microbiome
Krona Plot
Viral Microbiome
Krona Plot

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.28%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0235
male female RSP13125

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.998C>G p.Pro333Arg missense variant moderate contig741 4416830

IGV: Start, Jump

G/C
NGS:
0.182
C90:
0.000

Variants (Select Genes of Interest)

PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
DXR-2

UniProt

c.431C>G p.Ala144Gly missense variant moderate contig380 287760

IGV: Start, Jump

G/C
NGS:
0.182
C90:
0.550
aPT4

UniProt

c.97T>C p.Tyr33His missense variant moderate contig121 2828753

IGV: Start, Jump

T/C
NGS:
0.439
C90:
0.000
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000
AAE1-2

UniProt

c.1102C>A p.His368Asn missense variant moderate contig81 210064

IGV: Start, Jump

C/A
NGS:
0.033
C90:
0.000
AAE1-2

UniProt

c.1118C>G p.Thr373Ser missense variant moderate contig81 210080

IGV: Start, Jump

C/G
NGS:
0.026
C90:
0.000
HDS-1

UniProt

c.1618A>G p.Ile540Val missense variant moderate contig1891 885936

IGV: Start, Jump

T/C
NGS:
0.099
C90:
0.861

Nearest genetic relatives (All Samples)

0 0.058 0.117 0.175 0.233
closely related moderately related distantly related
  1. 0.188 Black Triangle (RSP11638)
  2. 0.203 Bueller F3 (RSP12503)
  3. 0.205 Fatso (RSP11741)
  4. 0.210 PK Drying (RSP13124)
  5. 0.210 Purple Urkle (RSP12890)
  6. 0.211 PK (RSP12932)
  7. 0.214 Skywalker OG (RSP10837)
  8. 0.215 PK (RSP13087)
  9. 0.215 East Coast Sour Diesel (RSP12922)
  10. 0.215 Rugburn OG (RSP11353)
  11. 0.216 Noetic OG (RSP11455)
  12. 0.217 RKM-2018-012 (RSP11103)
  13. 0.218 PK (RSP12926)
  14. 0.218 DB Dried (RSP13119)
  15. 0.218 SFVxTK (RSP11072)
  16. 0.219 Deadhead OG (RSP11463)
  17. 0.220 RKM-2018-013 (RSP11104)
  18. 0.220 Rootbeer Rolex 50 (RSP12645)
  19. 0.223 DB (RSP13083)
  20. 0.224 DB (RSP12930)

Most genetically distant strains (All Samples)

0 0.133 0.267 0.400 0.533
closely related moderately related distantly related
  1. 0.507 Jamaican Lion (RSP12916)
  2. 0.495 Cherry Blossom (RSP11306)
  3. 0.493 JL yellow (RSP11075)
  4. 0.492 Jamaican Lion (RSP12917)
  5. 0.488 Brunswick High (RSP11164)
  6. 0.483 Jamaican Lion (RSP12915)
  7. 0.481 Tanao Sri 46 (RSP11486)
  8. 0.473 Swaziland (SRR14708271)
  9. 0.471 JL X NSPM1 12 (RSP11472)
  10. 0.470 JL 3rd Gen Mother (RSP11214)
  11. 0.469 Cherry Blossom (RSP11298)
  12. 0.467 Cherry Blossom (RSP11309)
  13. 0.466 Cherry Blossom (RSP11326)
  14. 0.465 Cherry Blossom (RSP11325)
  15. 0.465 Cherry Blossom (RSP11322)
  16. 0.462 JL 4th Gen 5 (RSP11199)
  17. 0.460 JL 4th Gen 4 (RSP11198)
  18. 0.460 Cherry Blossom (RSP11329)
  19. 0.457 JL 4th Gen 6 (RSP11200)
  20. 0.456 Unknown- Cherry Wine - 003 (RSP11270)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4448643
Overlapping SNPs:
81
Concordance:
57

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495202
Overlapping SNPs:
1
Concordance:
1

Blockchain Registration Information

Transaction ID
a960ab58c0cfba255d4e76e9f06ed7b440f53adec0ceb8586d272407206c41b9
Stamping Certificate
Download PDF (39.5 KB)
SHASUM Hash
c72a5a50ad0469abaac1eb9041c46c6a91194d32bfedc05cf290f9fa4ee3d7c2
QR code for RSP13125

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