CDSD

RSP 12935

Grower: Zamir Punja

General Information

Sample Name
CDSD - roots from flowering plant
Accession Date
September 27, 2023
Reported Plant Sex
Female
Microbiome
Krona Plot
Fungal Microbiome
Krona Plot
Bacterial Microbiome
Krona Plot
Viral Microbiome
Krona Plot

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.2%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0276
male female RSP12935

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.749C>A p.Ala250Asp missense variant moderate contig741 4417079

IGV: Start, Jump

G/T
NGS:
0.127
C90:
0.632

Variants (Select Genes of Interest)

FAD2-2

UniProt

c.161T>A p.Leu54His missense variant moderate contig83 1803208

IGV: Start, Jump

A/T
NGS:
0.156
C90:
0.000
FAD2-2

UniProt

c.154G>A p.Val52Ile missense variant moderate contig83 1803215

IGV: Start, Jump

C/T
NGS:
0.022
C90:
0.000
FAD2-2

UniProt

c.62C>T p.Pro21Leu missense variant moderate contig83 1803307

IGV: Start, Jump

G/A
NGS:
0.042
C90:
0.000
ELF3

UniProt

c.358G>A p.Gly120Arg missense variant moderate contig97 242064

IGV: Start, Jump

G/A
NGS:
0.099
C90:
0.493
ELF3

UniProt

c.812G>C p.Gly271Ala missense variant moderate contig97 242518

IGV: Start, Jump

G/C
NGS:
0.114
C90:
0.938
ELF3

UniProt

c.1630A>G p.Thr544Ala missense variant moderate contig97 244461

IGV: Start, Jump

A/G
NGS:
0.103
C90:
0.545
ELF3

UniProt

c.2141C>G p.Pro714Arg missense variant moderate contig97 244972

IGV: Start, Jump

C/G
NGS:
0.088
C90:
0.335
ELF3

UniProt

c.2198G>T p.Arg733Leu missense variant moderate contig97 245029

IGV: Start, Jump

G/T
NGS:
0.125
C90:
0.531
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
PHL-1

UniProt

c.2551A>G p.Thr851Ala missense variant moderate contig1439 1487246

IGV: Start, Jump

T/C
NGS:
0.116
C90:
0.890
FLD

UniProt

c.125G>A p.Ser42Asn missense variant moderate contig1450 2047909

IGV: Start, Jump

C/T
NGS:
0.037
C90:
0.388
PIE1-1

UniProt

c.1222C>G p.Gln408Glu missense variant moderate contig1225 2281482

IGV: Start, Jump

C/G
NGS:
0.096
C90:
0.852

Nearest genetic relatives (All Samples)

0 0.067 0.133 0.200 0.267
closely related moderately related distantly related
  1. 0.111 CDSD (RSP12929)
  2. 0.196 Skywalker OG (RSP10837)
  3. 0.196 SFVxTK (RSP11072)
  4. 0.204 Super Runtz (RSP12810)
  5. 0.205 RKM-2018-026 (RSP11118)
  6. 0.206 Red Eye OG (RSP11190)
  7. 0.212 RKM-2018-013 (RSP11104)
  8. 0.214 Rootbeer Rolex 50 (RSP12645)
  9. 0.214 East side OG (RSP12089)
  10. 0.218 East Coast Sour Diesel (RSP12922)
  11. 0.224 Noetic OG (RSP11455)
  12. 0.225 Gorilla Cookies (RSP11231)
  13. 0.226 Cherry Lime Runtz (RSP12486)
  14. 0.229 Purple Urkle (RSP12890)
  15. 0.230 Fatso (RSP11741)
  16. 0.232 Eucalyptus (RSP12803)
  17. 0.232 Garlic (RSP11341)
  18. 0.239 RKM-2018-012 (RSP11103)
  19. 0.241 Champelli Red 4 (RSP12804)
  20. 0.243 Muscovite (RSP12809)

Most genetically distant strains (All Samples)

0 0.133 0.267 0.400 0.533
closely related moderately related distantly related
  1. 0.512 Cherry Blossom (RSP11306)
  2. 0.498 Cherry Blossom (RSP11318)
  3. 0.492 Cherry Blossom (RSP11325)
  4. 0.486 Cherry Blossom (RSP11322)
  5. 0.486 Cherry Blossom (RSP11329)
  6. 0.482 Cherry Blossom (RSP11315)
  7. 0.479 Tanao Sri 46 (RSP11486)
  8. 0.477 Cherry Blossom (RSP11326)
  9. 0.477 Northern Skunk (RSP11456)
  10. 0.470 JL X NSPM1 12 (RSP11472)
  11. 0.469 Cherry Blossom (RSP11300)
  12. 0.464 Wife (RSP11148)
  13. 0.462 Cherry Blossom (RSP11323)
  14. 0.460 Brunswick High (RSP11164)
  15. 0.459 Ringo s Gift Katie s Cut (RSP11624)
  16. 0.459 Cherry Blossom (RSP11316)
  17. 0.458 Cherry Blossom (RSP11319)
  18. 0.456 Cherry Blossom (RSP11302)
  19. 0.456 Unknown- Cherry Wine - 002 (RSP11269)
  20. 0.456 JL x NSPM1 4 (RSP11482)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8349389
Overlapping SNPs:
68
Concordance:
49

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495203
Overlapping SNPs:
14
Concordance:
10

Blockchain Registration Information

Transaction ID
772c8b8c9b88492c185ebbbac391a5f72b99bcea2cdc07806897bf4b980a29c0
Stamping Certificate
Download PDF (39.5 KB)
SHASUM Hash
a9c9e8695280c791114e4587a99f5a6c42b508ce6b4f84e0c25a00e7e731f660
QR code for RSP12935

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