Grape Diamonds

RSP 11724

Grower: Happy Valley Ventures

Summary

Grape Diamonds (RSP11724/Happy Valley Ventures) is a genetic Type I plant and its genetics appear rare. The sample's closest relative in the database is Domnesia (RSP11184/NETA) and it is a potential sibling. The heterozygosity rate is 0.97% which is average (38.0 percentile).

General Information

Accession Date
December 9, 2020
Reported Plant Sex
Female
DNA Extracted From
Stem

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 0.97%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0429
male female RSP11724

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.749C>A p.Ala250Asp missense variant moderate contig741 4417079

IGV: Start, Jump

G/T
NGS:
0.127
C90:
0.632

Variants (Select Genes of Interest)

PKSG-4a

UniProt

c.217C>T p.His73Tyr missense variant moderate contig700 1937628

IGV: Start, Jump

C/T
NGS:
0.000
C90:
0.000
PKSG-4a

UniProt

c.218A>T p.His73Leu missense variant moderate contig700 1937629

IGV: Start, Jump

A/T
NGS:
0.000
C90:
0.000
PKSG-4a

UniProt

c.224G>A p.Ser75Asn missense variant moderate contig700 1937635

IGV: Start, Jump

G/A
NGS:
0.000
C90:
0.000
PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.774G>A p.Met258Ile missense variant moderate contig700 1950864

IGV: Start, Jump

C/T
NGS:
0.366
C90:
0.947
PKSG-2b

UniProt

c.718T>A p.Phe240Ile missense variant moderate contig700 1950920

IGV: Start, Jump

A/T
NGS:
0.114
C90:
0.000
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-2b

UniProt

c.-2_1dupATA start lost & conservative inframe insertion high contig700 1951880

IGV: Start, Jump

A/ATAT
NGS:
0.410
C90:
0.000
PKSG-4b

UniProt

c.353_354insCC p.Gly119fs frameshift variant high contig700 2721319

IGV: Start, Jump

T/TGG
NGS:
0.175
C90:
0.000
DXR-2

UniProt

c.1319T>C p.Ile440Thr missense variant moderate contig380 285250

IGV: Start, Jump

A/G
NGS:
0.480
C90:
0.000
FAD2-2

UniProt

c.196T>C p.Phe66Leu missense variant moderate contig83 1803173

IGV: Start, Jump

A/G
NGS:
0.033
C90:
0.000
FAD2-2

UniProt

c.172G>T p.Asp58Tyr missense variant moderate contig83 1803197

IGV: Start, Jump

C/A
NGS:
0.070
C90:
0.000
FAD2-2

UniProt

c.161T>A p.Leu54His missense variant moderate contig83 1803208

IGV: Start, Jump

A/T
NGS:
0.156
C90:
0.000
aPT4

UniProt

c.97T>C p.Tyr33His missense variant moderate contig121 2828753

IGV: Start, Jump

T/C
NGS:
0.439
C90:
0.000
aPT4

UniProt

c.153A>C p.Lys51Asn missense variant moderate contig121 2828809

IGV: Start, Jump

A/C
NGS:
0.375
C90:
0.000
aPT4

UniProt

c.775delT p.Tyr259fs frameshift variant high contig121 2831380

IGV: Start, Jump

AT/A
NGS:
0.224
C90:
0.000
aPT4

UniProt

c.1168T>C p.Tyr390His missense variant moderate contig121 2833503

IGV: Start, Jump

T/C
NGS:
0.546
C90:
0.000
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000
HDS-1

UniProt

c.1381G>A p.Asp461Asn missense variant moderate contig1891 886367

IGV: Start, Jump

C/T
NGS:
0.029
C90:
0.000
HDS-1

UniProt

c.1378G>A p.Val460Ile missense variant moderate contig1891 886370

IGV: Start, Jump

C/T
NGS:
0.167
C90:
0.000
HDS-1

UniProt

c.-108+1_-108+2insG splice donor variant & intron variant high contig1891 889975

IGV: Start, Jump

A/AC
NGS:
0.217
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.067 0.133 0.200 0.267
closely related moderately related distantly related
  1. 0.185 Domnesia (RSP11184)
  2. 0.188 Super Lemon Haze (RSP11641)
  3. 0.207 Lime OG (RSP12101)
  4. 0.221 Blue Dream (RSP11009)
  5. 0.223 BLACK JACK (RSP11346)
  6. 0.224 Serious Happiness (RSP10763)
  7. 0.226 Black Jack (RSP10603)
  8. 0.229 RKM-2018-025 (RSP11117)
  9. 0.229 Durban Poison #1 (RSP10996)
  10. 0.229 Lift (RSP11378)
  11. 0.231 Top 44 (SRR14708268)
  12. 0.232 Blue Dream (RSP11010)
  13. 0.233 RKM-2018-016 (RSP11108)
  14. 0.233 Trump x Trump (RSP11466)
  15. 0.234 Durban Poison #1 (RSP11013)
  16. 0.234 Durban Poison (RSP10998)
  17. 0.235 Banana Daddy 1 -Big- (RSP11496)
  18. 0.236 Electra (RSP11366)
  19. 0.236 Super Blue Dream (RSP11011)
  20. 0.236 Blue Dream (RSP11007)

Most genetically distant strains (All Samples)

0 0.117 0.233 0.350 0.467
closely related moderately related distantly related
  1. 0.449 Cherry Blossom (RSP11323)
  2. 0.433 Cherry Blossom (RSP11311)
  3. 0.422 Cherry Blossom (RSP11317)
  4. 0.421 Cherry Blossom (RSP11318)
  5. 0.420 Cherry Blossom (RSP11328)
  6. 0.419 80E (RSP11213)
  7. 0.418 Candy Kush (RSP11492)
  8. 0.418 Cherry Blossom (RSP11314)
  9. 0.415 Cherry Blossom (RSP11335)
  10. 0.414 Cherry Blossom (RSP11334)
  11. 0.413 Cherry Blossom (RSP11308)
  12. 0.412 Ruderalis Indica (SRR14708267)
  13. 0.410 Cherry Blossom (RSP11309)
  14. 0.409 Unknown--Cherry Wine---003- (RSP11270)
  15. 0.408 R1in136 (SRR14708227)
  16. 0.406 Big Bud (SRR14708270)
  17. 0.406 XBL1 (SRR14708207)
  18. 0.406 Cherry Blossom (RSP11312)
  19. 0.404 BagSeed (RSP12627)
  20. 0.404 Feral (RSP11206)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8346480
Overlapping SNPs:
75
Concordance:
44

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495166
Overlapping SNPs:
2
Concordance:
2

Blockchain Registration Information

Transaction ID
8d6dff958a061975e93fcd3cc3f5958b524ac6d4c2128b15dc2ff3f73d3c7000
Stamping Certificate
Download PDF (39.4 KB)
SHASUM Hash
d8f738f47a2e36b8d48b1da77c5ee66d5bc99986439dec45c4ab463424d00997
QR code for RSP11724

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