OR_05MAY2017

RSP 10940

Grower: DigiPath Labs

General Information

Accession Date
May 4, 2017
Reported Plant Sex
not reported
DNA Extracted From
Unknown

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 0.95%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0349
male female RSP10940

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

No variants to report

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.419A>G p.Asp140Gly missense variant moderate contig700 2721254

IGV: Start, Jump

T/C
NGS:
0.430
C90:
0.440
aPT4

UniProt

c.775delT p.Tyr259fs frameshift variant high contig121 2831380

IGV: Start, Jump

AT/A
NGS:
0.224
C90:
0.000
aPT4

UniProt

c.1168T>C p.Tyr390His missense variant moderate contig121 2833503

IGV: Start, Jump

T/C
NGS:
0.546
C90:
0.000
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.050 0.100 0.150 0.200
closely related moderately related distantly related
  1. 0.139 White Chronic (RSP11220)
  2. 0.167 Blue Dream (RSP11004)
  3. 0.170 Blue Dream (RSP11010)
  4. 0.172 Blue Dream (RSP11017)
  5. 0.175 UP Sunrise (RSP10989)
  6. 0.178 Blue Dream (RSP11009)
  7. 0.181 Blue Dream (RSP11006)
  8. 0.181 Gold Cracker (RSP11048)
  9. 0.182 Gold Cracker (RSP11041)
  10. 0.184 Blue Dream (RSP11007)
  11. 0.185 Super Blue Dream (RSP11011)
  12. 0.189 Dave Pineapple (RSP11626)
  13. 0.191 RKM-2018-009 (RSP11100)
  14. 0.192 Serious Happiness (RSP10763)
  15. 0.192 Colombian 8 (SRR14708214)
  16. 0.192 Blue Dream (RSP11008)
  17. 0.193 Blue Dream (RSP11342)
  18. 0.193 Italian Kiss (RSP11034)
  19. 0.196 Up Sunset (RSP11256)
  20. 0.196 Escape Velocity (RSP11165)

Most genetically distant strains (All Samples)

0 0.125 0.250 0.375 0.500
closely related moderately related distantly related
  1. 0.494 Cherry Blossom (RSP11311)
  2. 0.475 Cherry Blossom (RSP11328)
  3. 0.451 Cherry Blossom (RSP11312)
  4. 0.447 Cherry Blossom (RSP11334)
  5. 0.445 Feral (RSP11205)
  6. 0.443 Unknown--Cherry Wine---003- (RSP11270)
  7. 0.437 Cherry Blossom (RSP11314)
  8. 0.436 Cherry Blossom (RSP11298)
  9. 0.434 Cherry Blossom (RSP11309)
  10. 0.428 Tiger Tail -30- (RSP11484)
  11. 0.424 Absolute OG (RSP11455)
  12. 0.422 Cherry Blossom (RSP11317)
  13. 0.422 Feral (RSP10890)
  14. 0.422 CS (RSP11208)
  15. 0.419 Cherry Blossom (RSP11300)
  16. 0.419 VIR 223 - Bernburgskaya Odnodomnaya - bm (SRR14708217)
  17. 0.418 Feral (RSP10891)
  18. 0.417 Unknown--Cherry Wine---001- (RSP11268)
  19. 0.416 R3in134 (SRR14708220)
  20. 0.414 Feral (RSP11206)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8346841
Overlapping SNPs:
73
Concordance:
60

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495248
Overlapping SNPs:
2
Concordance:
2

Blockchain Registration Information

Transaction ID
9d058b3a42dbf43fcf83d1b72fbddfcc5b9064ea66468e36b605c17db5663374
Stamping Certificate
Download PDF (859.3 KB)
SHASUM Hash
3e59c103a5a9d6068b6adf22f1d049326dac8f7a0d582c0c85bdb6040d8c4eda
QR code for RSP10940

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