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Stormy

RSP 11454

Grower: Blue Forest Farms

General Information

Sample Name
#5
Accession Date
March 31, 2020
Reported Plant Sex
Female
DNA Extracted From
Stem

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type III

File Downloads

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.23%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0517
male female RSP11454

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
CBDAS c.221C>G p.Thr74Ser missense variant moderate contig1772 2082447

IGV: Start, Jump

C/G
NGS:
0.136
C90:
0.057

Variants (Select Genes of Interest)
PKSG-4a

UniProt

c.493G>A p.Gly165Ser missense variant moderate contig700 1937904

IGV: Start, Jump

G/A
NGS:
0.103
C90:
0.000
PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.934C>G p.His312Asp missense variant moderate contig700 1950704

IGV: Start, Jump

G/C
NGS:
0.410
C90:
0.000
PKSG-4b

UniProt

c.556A>G p.Arg186Gly missense variant & splice region variant moderate contig700 2721117

IGV: Start, Jump

T/C
NGS:
0.022
C90:
0.000
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.352_355delACAG p.Thr118fs frameshift variant high contig700 2721317

IGV: Start, Jump

CCTGT/C
NGS:
0.529
C90:
0.000
DXR-2

UniProt

c.1319T>C p.Ile440Thr missense variant moderate contig380 285250

IGV: Start, Jump

A/G
NGS:
0.480
C90:
0.000
aPT4

UniProt

c.744+1_744+19delGTAATTTATTTTATTATAA splice donor variant & splice region variant & intron variant high contig121 2831022

IGV: Start, Jump

GGTAATTTATTTTATTATAA/G
NGS:
0.096
C90:
0.000
aPT4

UniProt

c.1168T>C p.Tyr390His missense variant moderate contig121 2833503

IGV: Start, Jump

T/C
NGS:
0.546
C90:
0.000
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.067 0.133 0.200 0.267
closely related moderately related distantly related
  1. 0.200 Trump x Trump (RSP11466)
  2. 0.202 Electra (RSP11366)
  3. 0.202 Doug s Varin (RSP11243)
  4. 0.209 Lift (RSP11378)
  5. 0.212 Queen Dream (RSP11289)
  6. 0.216 Durban Poison #1 (RSP11013)
  7. 0.216 Serious Happiness (RSP10763)
  8. 0.220 Rest (RSP11377)
  9. 0.222 Badger (RSP11614)
  10. 0.222 Domnesia (RSP11184)
  11. 0.223 Queen Dream (RSP11288)
  12. 0.227 Queen Dream CBG (RSP11295)
  13. 0.227 Durban Poison #1 (RSP10996)
  14. 0.231 Queen Dream CBG (RSP11280)
  15. 0.234 Queen Dream CBG (RSP11294)
  16. 0.235 Queen Dream CBG (RSP11284)
  17. 0.237 Joy (RSP11380)
  18. 0.241 Cherry Blossom (RSP11320)
  19. 0.241 NSPM x NSPM (RSP11487)
  20. 0.241 Lifter (RSP11365)

Most genetically distant strains (All Samples)

0 0.117 0.233 0.350 0.467
closely related moderately related distantly related
  1. 0.446 Chematonic -Cannatonic x Chemdawg- (RSP11394)
  2. 0.436 JL yellow (RSP11075)
  3. 0.421 Tanao Sri -46- (RSP11486)
  4. 0.419 Cherry Blossom (RSP11328)
  5. 0.411 RKM-2018-024 (RSP11116)
  6. 0.405 Ringo s Gift -Katie s Cut- (RSP11624)
  7. 0.405 JL 3rd Gen Mother (RSP11214)
  8. 0.405 JL 4th Gen 5 (RSP11199)
  9. 0.403 Cherry Blossom (RSP11318)
  10. 0.401 80E (RSP11213)
  11. 0.396 JL 4th Gen 2 (RSP11194)
  12. 0.392 Wife (RSP11148)
  13. 0.391 JL#2 (RSP11076)
  14. 0.388 R1in136 (SRR14708237)
  15. 0.387 80E (RSP11212)
  16. 0.387 Cherry Blossom (RSP11306)
  17. 0.387 R1in136 (SRR14708226)
  18. 0.385 JL 3rd Gen Mother (RSP11197)
  19. 0.385 Up Sunset (RSP11256)
  20. 0.384 Cherry Blossom (RSP11325)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4448597
Overlapping SNPs:
68
Concordance:
42

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495180
Overlapping SNPs:
4
Concordance:
4

Blockchain Registration Information

Transaction ID
5430f4ca360ef2bba7f1728a99ed2514d0815f06d18d77c5b9278e703afc2856
Stamping Certificate
Download PDF (39.9 KB)
SHASUM Hash
d8e7a71e9971f80582f88a9a64ca73612215675eac05dfa9752b29605e42955b
QR code for RSP11454

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