Pennywise

RSP 12904

Grower: Zamir Punja

General Information

Accession Date
August 23, 2023
Reported Plant Sex
Female
DNA Extracted From
Root
HLVd susceptible
yes
HLVd qPCR results
Detected in Root at CT 16.5; Leaf at CT 20.2
Microbiome
Krona Plot
Fungal Microbiome
Krona Plot
Bacterial Microbiome
Krona Plot
Viral Microbiome
Krona Plot

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type II

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.09%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0265
male female RSP12904

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

No variants to report

Variants (Select Genes of Interest)

PHL-2 c.3209A>G p.Gln1070Arg missense variant moderate contig2621 343252

IGV: Start, Jump

A/G
NGS:
0.103
C90:
0.885
FAD2-2

UniProt

c.64G>T p.Ala22Ser missense variant moderate contig83 1803305

IGV: Start, Jump

C/A
NGS:
0.048
C90:
0.000
ELF3

UniProt

c.1466G>A p.Ser489Asn missense variant moderate contig97 244297

IGV: Start, Jump

G/A
NGS:
0.123
C90:
0.000
ELF3

UniProt

c.1966C>G p.Pro656Ala missense variant moderate contig97 244797

IGV: Start, Jump

C/G
NGS:
0.123
C90:
0.632
Edestin

UniProt

c.16T>C p.Ser6Pro missense variant moderate contig850 3065274

IGV: Start, Jump

A/G
NGS:
0.059
C90:
0.000
EMF2

UniProt

c.1772A>G p.Gln591Arg missense variant moderate contig954 3059929

IGV: Start, Jump

A/G
NGS:
0.092
C90:
0.876
AAE1-3

UniProt

c.382T>C p.Tyr128His missense variant moderate contig976 1083643

IGV: Start, Jump

A/G
NGS:
0.072
C90:
0.000
PIE1-1

UniProt

c.1222C>G p.Gln408Glu missense variant moderate contig1225 2281482

IGV: Start, Jump

C/G
NGS:
0.096
C90:
0.852
PIE1-1

UniProt

c.1249G>T p.Val417Leu missense variant moderate contig1225 2281509

IGV: Start, Jump

G/T
NGS:
0.020
C90:
0.096
PKSB-3

UniProt

c.1901C>G p.Ala634Gly missense variant moderate contig93 3340008

IGV: Start, Jump

C/G
NGS:
0.059
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.067 0.133 0.200 0.267
closely related moderately related distantly related
  1. 0.098 PWE (RSP11369)
  2. 0.216 Tangerine Haze (RSP10995)
  3. 0.222 UP Sunrise (RSP10989)
  4. 0.228 Blue Dream (RSP11010)
  5. 0.228 Dave Pineapple (RSP11626)
  6. 0.228 Jacks Cleaner (RSP11347)
  7. 0.234 Green Crack (RSP11339)
  8. 0.238 Blue Dream (RSP11007)
  9. 0.238 Escape Velocity (RSP11165)
  10. 0.239 Blue Dream (RSP11017)
  11. 0.239 Durban Poison 1 (RSP11013)
  12. 0.239 RKM-2018-025 (RSP11117)
  13. 0.240 Calm (RSP11379)
  14. 0.240 RKM-2018-016 (RSP11108)
  15. 0.242 Saint Jack (RSP11179)
  16. 0.246 BLACK JACK (RSP11346)
  17. 0.247 Electra (RSP11366)
  18. 0.247 Blue Dream (RSP11004)
  19. 0.249 Durban Poison (RSP11014)
  20. 0.250 Black Jack (RSP10603)

Most genetically distant strains (All Samples)

0 0.117 0.233 0.350 0.467
closely related moderately related distantly related
  1. 0.457 Feral (RSP11205)
  2. 0.454 Fatso (RSP11741)
  3. 0.444 Cherry Blossom (RSP11314)
  4. 0.443 RKM-2018-012 (RSP11103)
  5. 0.441 Right Mark (RSP11628)
  6. 0.440 Bueller F3 (RSP12503)
  7. 0.439 GMO x Garlic Breath (RSP12507)
  8. 0.439 Cherry Blossom (RSP11309)
  9. 0.438 Red Eye OG (RSP11190)
  10. 0.437 Cherry Blossom (RSP11328)
  11. 0.435 Tanao Sri-white-T1 (RSP11658)
  12. 0.434 RKM-2018-026 (RSP11118)
  13. 0.432 East side OG (RSP12089)
  14. 0.432 Cherry Blossom (RSP11317)
  15. 0.431 Garlic (RSP11341)
  16. 0.430 Skywalker OG (RSP10837)
  17. 0.430 Rootbeer Rolex 50 (RSP12645)
  18. 0.429 R3in134 (SRR14708220)
  19. 0.429 Torrey (RSP11449)
  20. 0.429 Peanut Butter Breath (RSP11640)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8349140
Overlapping SNPs:
53
Concordance:
37

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495185
Overlapping SNPs:
5
Concordance:
5

Blockchain Registration Information

Transaction ID
7076c60676a34735eb34992db492b3cbb3663c02009e76967b8a93fb2a558f52
Stamping Certificate
Download PDF (39.5 KB)
SHASUM Hash
3a6e3af24b53d8ea782d0b33ec0290aa229357307867d5cfe9bc2ad970c050ae
QR code for RSP12904

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